파장 정확도 : < ±0. BMG Labtech - THERMOstar. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and. Tristar 3 Multimode Microplate Reader. All parts of the SpectraTest ABS1 Validation Plate are warranted for a period of ninety (90) days from the original date of delivery. Find regional support. Data were analyzed with GraphPad Prism. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. A. Windows Home Editions are not supported. Supplier: Molecular Devices. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The absorbance value (OD) was measured at 492 nm with a microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA). Wavelength Range. Florescence intensity was monitored on a Gemini XPS fluorescence microplate reader (Molecular Devices, Sunnyvale, CA, USA) set for 488 nm excitation and 520 nm emission. Reboot the computer. Narrow bandwidths deliver increased accuracy and enhanced peak. The optical density (OD) was measured at 570 nm (with a reference at 690 nm) by UV-visible spectrophotometer (VersaMax, Molecular Devices, CA, USA) in triplicates. ¨ Molecular Devices is a life-science technology innovator Our integrated hardware and software solutions enable automated workflows—from start to finish—for any research application. Concentration was determined by BCA Protein Assay Kit (ref. . VersaMax,SpectraMax340PC384,190,Plus384MicroplateReadersUserGuide 11 0112-0126B Theguidelinesforcuvetteuseintheinstrumentarethesameasthosethatapplytoany high. The plates were incubated at RT for an additional 25 min to stabilize the luminescence signals followed by the measurement of luminescence using a microplate reader (VersaMax, Molecular Devices, CA, USA) (n = 6). Electrolyte leakage analysis was conducted using 8-day-old rice seedlings before and after cold stress treatments in different time points (0, 6, and 11 days) at 4°C. Our absorbance plate readers feature our PathCheck Sensor technology and industry-leading SoftMax® Pro Data Acquisition and Analysis Software. 5 × 10 6 cells per well inoculated into 6-well plates in culture medium with CSF1 (10 ng/mL) for 7. The SpectraTest® ABS1 Absorbance Validation. For quantitative analysis, the stains were re-dissolved in isopropanol, and the absorbances were measured at 520 nm using a spectrophotometer (VersaMax, Molecular Devices, CA, USA). , Sunnyvale, CA, USA). The quantity of nitrite in the samples was calculated using the concentration of sodium nitrite as a standard. This. With optimized reagents, option to operate the. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. HepG2 cells were seeded into 6-well plates. Ensure data accuracy of your reader. Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the VERSAmax Tunable Microplate Reader are warranted for a period of one (1) year from the original date of delivery. Absorbance was determined at 490 nm on a microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). Narrow bandwidths deliver increased accuracy and enhanced peak resolution. Regular price $ 0. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. GABase was a mixture of γ-aminobutyrate glutamate amino transferase. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The. VERSA MAX-LUNABLE, MOLECULAR DEVICES, MICROPLATE R. spectrophotometer (VERSAmax, Molecular Devices). VERSAMAX. 00. 9. Softmax Pro 소프트웨어가 포함된 PC 워크스테이션 포함. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm pathlength cuvette. We would like to show you a description here but the site won’t allow us. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. The absorbance was measured at 540 nm using a hybrid microplate reader (VersaMax; Molecular Devices, Sunnyvale, CA, USA). We would like to show you a description here but the site won’t allow us. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. After washing in PBS at least 4 times to remove all extracellular dye, samples were fixed using 4 % PFA/PBS for. DISCLAIMER Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. 0, 0. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. Briefly, at 3 h before each of the desired time points, 10 µl of the MTS reagent. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. Molecular Devices Corporation reserves the right to change its products and services at any time to incorporate technological developments. 0%, 0-2. Measurement of brain-derived neurotrophic factor (BDNF) release. The. We would like to show you a description here but the site won’t allow us. Indocyanine Green Depuration Assay. SpectraTest® Validation Package. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. used. SpectraTest ABS1 Absorbance Validation Plate. If you are looking for a comprehensive user guide for SoftMax Pro 7. The Molecular Devices VersaMax ELISA Microplate Reader enables precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing. 4. Oil red O staining. +86-21-6887-8820 fax +86-21-6887-8890 Germany Molecular Devices GmbH Tel. RT-qPCR. Molecular Devices versamax Operator's Manual (47 pages) Tunable Microplate Reader. The emodin thermoreversible PG was. , Sunnyvale, USA). 00 Sale price $ 0. g. VersaMax Absorbance Microplate Reader memungkinkan pemilihan hingga dua panjang gelombang sekaligus untuk deteksi absorbansi dalam. The average OD 490 of 12 blank wells (Line 18) was then subtracted from the raw OD 490 obtained for each sample well. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. Invasion assayCells were seeded into the top chamber of a 96-well matrigel-coated plate with 8-m-pore polyethylene terephthalate membrane inserts (Corning). DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. The rate of cell viability was calculated using the following formula:The conversion of NADP+ to NADPH was measured as absorbance (340 nm) on a microplate reader (VersaMax, Molecular Devices Co. 9000-0756. Table 1 Conditions for biofilm formation on lids of 96-pin microtiter plates. 17. For more sample throughput, the system can be easily integrated into a full robotic The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. StakMax. Order Now. DMEM medium supplemented with the same volume of kit reagent on an empty well was used as a blank. Products : General, Microplate Readers. Microplate assays involved a 96-microplate reader (VERSAmax; Molecular devices, Sunnydale, California, USA) used with flat-bottomed 96-well microplates (NUNC, Sigma Aldrich, UK). S. Turn the reader on and secure the USB cable. Afterward, HUVECs were incubated with EPC-EVs, TFEB-EVs, or PBS for 24 h or 48 h. BMG Labtech - THERMOstar. Molecular Devices SpectraMax Plus 384 Microplate Reader *Parts Only* Tecan Sunrise Microplate Reader. Battery Backup Uninterruptible Power Supply (UPS) And Power Conditioner For MDS Sciex Molecular Devices VERSAmax Microplate Reader Quote Request. treatments were then examined atλ=570 nm using VersaMax eliSa reader (Molecular devices, llc. ® SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Bacterial growth was determined turbimetrically using the microplate ELISA reader VERSAmax (Molecular Devices). Optical Densitiy (OD) values were processed with a data reduction software (SoftMax Pro, Molecular Devices) using a 4-parameter logistic fitting algorithm to the standard curve, allowing the determination of antibody. absorbance readers. MOLECULAR DEVICES VersaMax is a customizable lab equipment capable of performing multiple types of research-grade tests and experiments at once, with enhanced safety protocols and adjustable column and sample-loading system for efficient sample preparation and increased throughput. The Organoid Innovation Center at Molecular Devices combines cutting-edge technologies with novel research methods to address key challenges of scaling complex 3D biological models. Concluding Remarks. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. At All-usermanuals. The optical densities (ODs) of the reactants were measured at 450 nm using a microplate spectrophotometer (Versamax, Molecular Devices, CA, USA). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer. The. 2. Add to Cart. Select Start>Control panel>Device Manager. SoftMax® Pro Software is the leading solution for microplate reader control and data analysis, where our exclusive INSIDER members help to ensure it remains the most innovative research tool available. 635. SpectraMax®, VersaMax, VMax. 5577; General Inquiry Form; Mon-Fri 8:00am - 5:00pmSpectraMax and VersaMax . The senescence-associated β -galactosidase activity (SA- β -gal) was measured using β -galactosidase Assay Kit following manufacturer's protocol. The assay was performed in 96-well microtiter plates in duplicate, and the absorbance was read at 405 nm with 620 nm as the reference wavelength using a microplate reader (VersaMax, Molecular Devices, Boston, Massachusetts). FAST AND ACCURATE METERTECH Microplate Reader $3,650. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. 3 cells (6x105) were ocExtracts were placed in a 96-well plate in triplets and spectrophotometric readings were taken at 555 nm on a microplate reader (Versamax, Molecular Devices LLC, USA). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. With its advanced features and user-friendly interface, the VersaMax is the perfect choice for a wide range of research. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The activity was measured at 40 °C following o-nitrophenol (ONP) release at 405 nm using a microplate reader (Versamax, Molecular Devices, San Jose, CA, USA). The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. 8), 40 μL of tyrosinase, and 40 μL of L-DOPA was put in each well. 747. This. New and used Molecular Devices VersaMax's for sale on LabX. The color was allowed to develop for 20 min in dark and the reaction was stopped with 2. The cellular viability. The system provides automation for up to 50For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. StakMax Microplate Handling System. 키네틱 읽기 시간 : 최소 9초 간격. They were then pre-treated with indicated concentration of drugs for 1 h prior to being treated or not with 5-HT or serum. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. MOLECULAR DEVICES VersaMax Reader: Model #: VersaMax: Serial #: N/A: Year Built: N/A: Location: California: Price: $3,800. 2 and 648. Molecular Devices公司的VersaMax读 板机为光栅型单色器读板机，拥有灵活检 测优势，无需加配额外的滤光片。 可调波长，无需滤光片，灵活 VersaMax读板机使用光栅单色器来选择 波长，其结果是选择确切的检测波长应用 于每个实验。波长范围从340nm到 With the VersaMax microplate reader, you will never have to buy another filter. DNA content in the supernatant (100 μl) was measured using a Quant-iT PicoGreen assay kit (Invitrogen) as per the manufacturer's instructions. Ann Arbor, MI, USA). 1 mL. 8 in. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. absorbance readers. Negative (phosphate buffered saline) (Gibco Waltham, MA, USA) and positive controls. 747. together with SpectraMax, VersaMax, VMax, and EMax microplate readers from Molecular Devices, offer a quality assurance screening solution for the detection of melamine contamination in such food products as milk, infant formula, pet food, and confectionery. And the VERSAmax reader is robotics-compatible. To make your request, nothing more simple: you just have to connect on the site and to make your request on line. 1. SpectraMax® Microplate Reader User Manual Download Page. All samples were measured in triplicate. A. ) gap between the rear of the instrument and the wall. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0140 Rev. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The amount of MTT reduction was measured immediately by detecting absorbance in a microplate reader (Versamax, Molecular Devices) at a wavelength of 570 nm and a reference wavelength. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. The Molecular Devices VersaMax Absorbance Microplate Reader enables both precise and accurate detection at wavelengths in the visible spectrum without the use of filters, providing consequence-free assay flexibility. 5% NP-40) supplemented with protease and phosphatase inhibitor cocktail (GenDepot, Baker, TX, USA). The increase of absorbance at 405. The percentage of cytotoxicity was calculated using the following formula: cytotoxicity (%) = [1 − (At/As) × 100], where At and As refer to the absorbance of a tested. For pinocytosis, cells in wells were washed in PBS and 5 mg/ml of HPTS dye (in PBS) added and incubated with gentle rocking at 30 °C for 120 min. Our specialization servicing microplate instrumentation and our focus on a narrow range of product lines allows us to provide the highest standards of service and ensure. The signal was developed using 3,3′, 5,5′-tetramethylbenzidine after streptavidin–peroxidase treatment. 0 out of 5 $ 23,995 $ 22,495. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. $199. The nuclear extracts were subsequently determined for the content of NF-κB with the NF-κB (p65) transcription factor assay kit. 99 shipping. The reaction in the wells was stopped by the addition of 50 μl of 1 M sulfuric acid, which turned the wells yellow. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Coat plate with 100 μL each of appropriately diluted standards (Table 1),. 0. S. The assay procedure was in accordance with the manufacturer's instructions, and the results were analyzed with a microplate reader (VersaMax; Molecular Devices Ltd. Measurement of biofilm formation inhibitory activity. ChE activity was calculated as the rate of change of absorbance per minute during the linear portion of the reaction. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Page 6 SpectraMax Plus384, 190, 340PC384, and VersaMax. In brief, mature 3T3-L1 cells were washed with DPBS, fixed with 10% formalin for 1 h at room temperature, washed once with 60%. A solution of 10 millimolar L-DOPA (40 µL) was added to each sample and the blank. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Results of host range test of Mae-Yong924-1 against. Youmustensurethatthemeniscusiscomfortablyabove thelightbeaminstandardcuvettesandthatthesamplechamberinamicrocuvetteis Microplate Reader Capability > UV - Vis Absorbance. 파장 범위(nm) : 340–850. An aliquot of the extracts dissolved in DMSO with 80 μL of phosphate buffer (pH 6. SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PMThis version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 3. 90 shipping. The plate was read at 570 nm after 5 min using Versamax (molecular devices, United States). 4. After waiting 15 minutes for colour development at room temperature, the reaction was stopped with 1 M H 2 SO 4, and optical density values were read using an ELISA reader (Versamax, Molecular Devices, LLC, USA) at a wavelength of 492 nm, and the results were given as OD values. The optical density of each well at 490 nm was then measured using a microplate spectrophotometer system (VersaMax; Molecular Devices, Sunnyvale, CA, USA). 99 + $59. 72 MB. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY $3,450. Preconfigured method protocols specifically written for the Pyrochrome Assay and other ACC assays are available for SoftMax Pro Software and take. The reaction was started by adding 10 µL of the enzyme solution (properly diluted) to 190 µL of 15 mM ONPG in 0. StakMax. Biosystems CytoFluor 4000 Multi Well Plate Reader $ 1,995. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. Phosphodiesterase isozymes: molecular targets for novel antiasthma agents. 0A-Sunnyvale, CA-Made in USA SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). , VersaMax; Molecular Devices) Incubator for cell culture. printed in u. Conventions Used in this Manual. Whether bacteria were partially or completely inactivated by bone cement. The blue formazan concentration formed in viable cells was measured at 570 nm using spectrophotometer (Versamax, Molecular Devices, São Paulo, Brazil). SpectraMax Plus384, 190, 340PC384, and VersaMax Installation Guide Author: Molecular Devices Subject: Installation Guide Keywords: SpectraMax Versa and others Detection Platform Created Date: 5/17/2023 2:14:27 PM At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. StakMax Microplate Handling Stacker. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Quantitative real-time polymerase chain reaction For quantitative real-time polymerase chain reaction (qRT-PCR) analysis, total cellular RNA was extracted from ten zebrafish larvae. After treatment, cells were collected, lysed with the lysis buffer. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Cell migration assay. The in vitro differentiation of BMDMs. #0120-1293A SpectraMax, SoftMax Pro, Vmax and Emax are registered trademarks and VersaMax, Lmax,Although this manual has been prepared with every precaution to ensure accuracy, Molecular Devices Corporation assumes no liability for any errors or omissions, nor for any damages resulting from the application or use of this information. The plate was read at 570 nm using an ELISA reader (VERSAMax, Molecular Devices, VWR, Philadelphia, PA, USA). 6 nm, respectively, by ELISA microplate reader (VERSAmax, Molecular Devices, USA). # SMP54-GXP- 10UL) Pyrochrome Assay. for cell lysis via visual inspection and optical microscopy (VersaMax, Molecular Devices, Sunnyvale, CA, USA) observation and checked by measuring the absorbance at OD680. 0 OD. Tris base (100 μL of a 10 mM solution) was added to the wells to solubilize the bound SRB, and absorbance was then read at 515 nm on an automated microplate reader (VERSAmax, Molecular Devices, Sunnyvale, CA, USA). Molecular devices Versa Max. 0A Sunnyvale, CA Made in USA Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). from Molecular Devices Corporation. Bimolecular fluorescence complementation (BiFC) systemMolecular Devices Versamax Tunable Microplate Reader is available from The Lab World Group. Table of Contents. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. 2 and 648. SpectraMax and VersaMax Plate Readers Operator s Manual -- 0112-0126 Rev A Molecular De. ® ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. The absorbance at wavelength 450 nm was detected using a micro-plate reader (VersaMax, Molecular Devices, USA). The detection limit for rivaroxaban in this assay was 10 ng/mL. Statistical analysis Graphs and statistics were prepared in GraphPad Prism, version 7. Flexible calculations Data analysis features are available such as 21 different curve fit options, simplified cross-plate analysis and interpolation, and automatic calculations. It features a modular design that allows users to add or change detection cartridges according to their needs. SpectraMax® i3 Multi-Mode Detection Platform. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. Data were normalized to those of the PBS exposure control group. SpectraMax®, VersaMax, VMax. Read-out was performed within 30 minutes with a microtiter plate reader at 450 nm (Versamax, Molecular Devices). VersaMax ELISA Microplate Reader from Molecular Devices, LLC Description Enables precise and accurate detection at wavelengths in the visible spectrum without the use of. 0. Our mission: find your user guide or user's manual as quickly as possible. 99 + $42. Shark chondroitin sulfate A sodium salt (Sigma Aldrich Corp. +1 800-635-5577. Spectranet customer portal. Lihat Video. These values were transformed to logarithmic scale to create a log-log curve. repurposing of drug molecules already approved, or in clinical trials, for other viruses. Flow Cytometry AnalysisAt the end of the exposure, the MTS solution (20 µL/well) was added, and the cells were incubated for 3 h at 37°C. The assays were conducted in a 96-well microplate using an ELISA microplate reader (VersaMax Molecular Devices, United States) to measure absorbance at 475 nm. Molecular Devices also offers a mass spectrometry-based solution for food safety testingWe would like to show you a description here but the site won’t allow us. For more advanced automation needs, Molecular Devices interacts with all of the major lab automation providers, and is one of their leading choices. The emodin thermoreversible PG was. TECHNICAL NOTE. The absorbance was assumed to be 100% for the control group. Glossary of Terms. It provides both rapid and sensitive measurements of a variety of analytes across a wide range of concentrations. The wells were visualized using o-phenylenediamine (OPD), and the absorbance was read with a multiwall spectrophotometer (VERSAmax, Molecular Devices, Sunnyvale, CA, USA) at 490 nm. Innovative and flexible design features enable accelerated sample preparation time and increased laboratory productivity of DNA, RNA, and protein samples. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial. Microplate Reader Shelf. Cet appareil ISM est confomre à la norme NMB-001 du Canada. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate. For more information, please visit us at With the VersaMax microplate reader, you will never have to buy another filter. Technical Specifications. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. Activity was calculated as picomoles of substrate hydrolyzed per min. 0A Sunnyvale, CA Made in USAVersaMax price. Supplier: Molecular Devices. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Cell proliferation assay MDA-MB-231 cells were seeded at a density. We would like to show you a description here but the site won’t allow us. The plates were immediately measured at 405 nm for 6 min using a microtiter plate reader (VersaMax; Molecular Devices, Sunnyvale). 72 MB. The catalytic activity was calculated from the production rate of the hydrolyzed product, p-nitrophenol, by monitoring the absorption (405 nm) at room temperature. We use. (VersaMax, Molecular Devices, USA). One microgram of RNA was. In this study, we have prepared thermoreversible PG containing emodin with high emodin solubility and the gelation temperature suited to physiological use. NORTH AMERICA. We would like to show you a description here but the site won’t allow us. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. The well plates were then washed with 1% acetic acid and dried. Color intensities were measured using microplate reader (Versamax, Molecular Devices, Inc. and California) Tunable Microplate Reader 100-240V ~ 4A 50-60 HZ T4. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. This version is compatible with the following instruments: SpectraMax® i3x Multi-Mode Detection Platform. Click SoftMax Pro Software User Guide. 4. TECHNICAL NOTE. After that, absornace were measured at 520 nm using using a microplate reader spectrophotometer VERSAmax (Molecular Devices). To purchase a newer software version, request a quote. Multichannel optics. The absorbance was detected at 517 nm wavelength using a microplate reader (VersaMax, Molecular devices, USA). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard. Louis, USA) was used to. 8). SpectraMax Plus384 SpectraMax 190 SpectraMax 340PC384 VersaMax Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. New and used Molecular Devices VersaMax's for sale on LabX. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Molecular Devices Spectramax Plate Reader. These models can no longer be repaired. The mouse hippocampal neuronal cell line HT22 is frequently used as an in vitro model to investigate the role of hippocampal cholinergic neurons in cognitive functions. Figure S2: The cellular localization of NDRG1 is not affected by N- or C- terminal deleted NDRG1 mutants under hypoxic conditions. We would like to show you a description here but the site won’t allow us. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Cells were treated with 150 μl of DMSO and absorbance was quantified in 540 nm using the microplate spectrophotometer system (VersaMax, Molecular Devices, Sunnyvale, CA, USA). 00 Regular price. A microplate reader (VersaMax, Molecular Devices, USA) was used to detect the OD value at 450 nm. Whether its cell line development, 3D biology, drug screening—or a combination of the three—our technology maximizes throughput and reproducibility to give. Select the Driver tab. Readings were taken every 15 s at 490 nm and the results were analysed using SOFT-MaxPro 4. 4. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Plates were read using a microplate reader (VersaMax, Molecular Devices) at 595 nm. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. All. Ensure data accuracy of your reader. 0 software (GraphPad). The absorbance was measured at 405 nm using a plate reader (VersaMax, Molecular Devices) (N = 3). . The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. S. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340–850nm). Molecular Devices VersaMax Tunable Temperature range between 4°C above ambient to 45°C Digital controller and display Sold as-is The Molecular Devices VersaMax. 45% of MTT reagent in cell culture media was substituted and incubated for 4 h before adding 100 μL of 100× DMSO. The absorbance was then determined by an enzyme-linked immunosorbent assay (ELISA) reader (VERSAmax; Molecular Devices, Sunnyvale, CA) at a wavelength of 405 nm. Molecular Devices Corporation. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Find regional support. The optical density was measured at a wavelength of 450 nm using VersaMax (Molecular Devices, Seoul, South Korea). +49-89/96-05-88-0 fax +49-89/9-62-02-34-5 Japan Nihon Molecular Devices Osaka Tel. HUVECs were seeded into 96-well plates at a density of 30,000 cells/well and allowed to attach for 12 h. Dual monochromatic, three modes detection, UV Vis Abs, Fluor, and Lum, cuvette. Used. It features a modular design that allows users to add or change detection cartridges according to their needs. Turn the reader on and secure the USB cable. Finally, 0. It features a dual-monochromator system, a large dynamic range, and a high-throughput capability. Briefly, 100 μl of cell culture medium was mixed with 100 μl of Griess reagent [equal volumes of 1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0. The limit of detection for each assay was 156. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Then the absorbance at 570 nm was measured using a microplate reader (VersaMax, Molecular Devices, San Jose, CA, USA). Use Windows Search to search for “Add or Remove”. Wavelength Accuracy. To test if sodium arsenite and dimethylarsenic acid could stimulate apoptosis in 3 cells, oc cell cycle redistribution was studied using flow cytometry with propidium iodide staining.